Zobrazit minimální záznam
dc.contributor.author |
Kanďár, Roman |
|
dc.contributor.author |
Žáková, Pavla |
|
dc.contributor.author |
Frölichová, Martina |
|
dc.contributor.author |
Štramová, Xenie |
|
dc.contributor.author |
Bencová, Soňa |
|
dc.date.accessioned |
2012-03-15T11:26:15Z |
|
dc.date.available |
2012-03-15T11:26:15Z |
|
dc.date.issued |
2010 |
|
dc.identifier |
Univerzitní knihovna (studovna) |
|
dc.identifier.isbn |
978-80-7395-386-7 (Online) |
|
dc.identifier.isbn |
978-80-7395-368-3 (Print) |
|
dc.identifier.issn |
1211-5541 |
|
dc.identifier.uri |
http://hdl.handle.net/10195/42633 |
|
dc.description.abstract |
The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the
intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 :g l–1, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0-51.6 :g l–1
88 Kanďár R. et al./Sci. Pap. Univ. Pardubice Ser. A 16 (2010) 87–98(0.59-3.20 :g mmol–1 creatinine) and 14.9-65.4 :g L-1 (0.98-8.21 :g mmol–1 creatinine). The presented method is inexpensive and suitable for screening of
porphyrias. |
eng |
dc.format |
p. 87-98 |
|
dc.language.iso |
eng |
|
dc.publisher |
Univerzita Pardubice |
cze |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 16 (2010) |
|
dc.subject |
porphyrins |
eng |
dc.subject |
HPLC method |
eng |
dc.subject |
coproporphyrin I |
eng |
dc.subject |
coproporphyrin III |
eng |
dc.title |
Determination of coproporphyrin I and III in human urine using HPLC with fluorescence detection |
eng |
dc.type |
Article |
eng |
dc.identifier.signature |
47333-16 |
|
dc.peerreviewed |
yes |
|
dc.publicationstatus |
published |
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