Abstract:
With the aim to minimize the amount of biological material needed for analysis,
a new type of sample preparation has been used for the determination of the
percentage content of fatty acids in human blood. The blood samples from the
volunteers (n = 30) were processed in two ways, as a plasma and as dried blood
spot (DBS) samples. The fatty acids were firstly derivatized into the respective
esters and, subsequently, separated and detected using gas chromatography (GC)
with a flame ionization detector (FID). The results obtained from the DBS sample
analysis were compared with those obtained by the established method for the
determination of fatty acids in plasma.
