Zobrazit minimální záznam
dc.contributor.author |
Murasová, Pavla
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dc.contributor.author |
Kovářová, Aneta
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dc.contributor.author |
Srbová, Jana
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dc.contributor.author |
Bílková, Zuzana
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dc.date.accessioned |
2018-10-10T12:04:32Z |
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dc.date.available |
2018-10-10T12:04:32Z |
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dc.date.issued |
2018 |
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dc.identifier.isbn |
978-80-7560-167-4 |
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dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/71963 |
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dc.description.abstract |
With the advent of micro- and nanotechnology, major advances and breakthroughs have
taken place in many areas, such as biotechnology, medicine, pharmacy or material
industry. Gold nanoparticles (AuNPs) with their unique optical and physical properties
as well as biocompatibility have already proved themselves to be powerful tool in
biological applications. In this work, AuNPs were selected as a potential
electrochemically active label. With the aim to use them as an electrochemical active
tag, two types of AuNPs were tested and suitable protocols for their functionalization
were optimized. Non-functionalized AuNPs were modified with thiolated DNA
oligonucleotides using Au-S bond. Such conjugates could be potentionally used as a
DNA probe. Functionalized AuNPs containing carboxyl groups were also modified, but
with specific antibodies. In this case, the goal was to optimize suitable protocol for
effective modification performed with carbodiimide crosslinker enabling direct and
covalent immobilization. Finally, quality of AuNPs-IgG was defined by experiments via
detection of bacterial cells in model samples. |
en |
dc.format |
p. 109 -118 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 24/2018 |
en |
dc.rights |
open access |
en |
dc.subject |
gold nanoparticles |
en |
dc.subject |
bioconjugation |
en |
dc.subject |
label |
en |
dc.subject |
immunosensor |
en |
dc.title |
Different approaches for preparation of AuNPs-based conjugates as a signal-generating unit for electrochemical biosensors |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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