New Interface for Purification of Proteins: 1D TiO2 Nanotubes Decorated by Fe3O4 nanoparticles

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dc.contributor.author Kupčík, Rudolf
dc.contributor.author Řehulka, Pavel
dc.contributor.author Bílková, Zuzana
dc.contributor.author Sopha, Hanna
dc.contributor.author Macák, Jan
dc.date.accessioned 2017-08-15T10:54:11Z
dc.date.available 2017-08-15T10:54:11Z
dc.date.issued 2017-08-08
dc.identifier.issn 1944-8244
dc.identifier.uri http://hdl.handle.net/10195/68975
dc.description.abstract In this work, a high surface area interface, based on anodic 1D TiO2 nanotubes homogenously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs) is reported for the first time for an unprecedented purification of His-tagged recombinant proteins. Excellent purification results were achieved from the model protein mixture, as well as from the whole cell lysate (with His-tagged ubiquitin). Compared to a conventional Immobilized-Metal Affinity Chromatography (IMAC) system, specific isolation of selected His-tagged proteins on behalf of other proteins was significantly enhanced on TiO2NTs@Fe3O4NP´s interface under optimized binding and elution conditions. The combination of specific isolation properties, magnetic features, biocompatibility, and ease of preparation of this material consisting of two basic metal oxides, makes it a suitable candidate for future purification of recombinant proteins in biotechnology. The principally new material bears a large potential to open new pathways for discoveries in nanobiotechnology and nanomedicine. cze
dc.format p. 1-41 eng
dc.language.iso en cze
dc.publisher American Chemical Society cze
dc.relation.ispartof ACS Applied Materials & Interfaces. 2017
dc.rights Attribution-NonCommercial-NoDerivs 3.0 Czech Republic *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/cz/ *
dc.subject self-organized TiO2 nanotubes cze
dc.subject magnetic nanoparticles cze
dc.subject recombinant His-tagged proteins cze
dc.subject isolation cze
dc.subject purification cze
dc.title New Interface for Purification of Proteins: 1D TiO2 Nanotubes Decorated by Fe3O4 nanoparticles cze
dc.type Article cze
dc.event
dc.peerreviewed yes eng
dc.publicationstatus postprint eng
dc.identifier.doi 10.1021/acsami.7b08445
dc.relation.publisherversion
dc.project.ID EC/H2020/638857/EU/Towards New Generation of Solid-State Photovoltaic Cell: Harvesting Nanotubular Titania and Hybrid Chromophores/CHROMTISOL


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Attribution-NonCommercial-NoDerivs 3.0 Czech Republic Kromě případů, kde je uvedeno jinak, licence tohoto záznamu je Attribution-NonCommercial-NoDerivs 3.0 Czech Republic

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