http://hdl.handle.net/10195/38121 2013-05-22T15:35:05Z 2013-05-22T15:35:05Z Chochorek, Aneta Bobrowski, Andrzej Mocák, Jan http://hdl.handle.net/10195/32754 2012-02-12T06:01:05Z 2006-01-01T00:00:00Z

Title: Estimation of uncert ainity of the ICP AES determination of selected metals in inland waters Authors: Chochorek, Aneta; Bobrowski, Andrzej; Mocák, Jan Abstract: Determination of basic metrological characteristics and estimation of the combined uncertainty and expanded uncertainty of the results of the ICP AES determination of ten metals (Zn, Cr, Cd, Cu, Ni, Pb, Fe, Mn, Mg and Ca) commonly present in inland waters were performed. Special attention was given to the assessment, which uncertainty components play a dominant role in the overall determination process (from sampling up to the presentation of results), and particularly, in the ICP AES calibration and measurement. The largest contribution to the combined uncertainty of the final analytical result in all ten analytical determinations was found from the sampling procedure.

2006-01-01T00:00:00Z Hejtmánková, Ludmila Sedlák, Miloš http://hdl.handle.net/10195/32751 2012-02-12T06:01:17Z 2006-01-01T00:00:00Z

Title: Recent advances in ring transformation of five-membered heterocycles Authors: Hejtmánková, Ludmila; Sedlák, Miloš Abstract: Ring transformations of heterocyclic rings by temporary opening and subsequent closure to a new molecule are of particular interest both synthetically and theoretically. Only rearrangement of five-membered heterocycles are discussed and reviewed. These transformations are classified into four groups: (a) "classical ring transformations", where the starting and resulting ring system is of the same size, but the heteroatoms and/or their positions have been changed: (b) "degenerate ring transformations" where during the course of the transformation the starting compound and product have the same ring system, but the reaction proceeds by a ring opening and subsequent ring closure process: (c) "ring contractions" and "ring expansions" where the sizes of the product rings are smaller or larger, respectively, than those of the starting compound, and (d) "pseudo ring transformations" or "ring-chain transfer", where the process is formally a ring transformation, but is realised by a ring closure of a side chain of the starting heterocycle and opening of the orirignal ring. Reaction mechanism of the most interesting ring transformations are also discussed.

2006-01-01T00:00:00Z Waryo, Tesfaye T. Begić, Sabina Turkusić, Emir Vytřas, Karel Kalcher, Kurt http://hdl.handle.net/10195/32748 2012-02-12T06:01:26Z 2006-01-01T00:00:00Z

Title: Fe3O4-modified thick film carbon-based amperometric oxidase-biosensor Authors: Waryo, Tesfaye T.; Begić, Sabina; Turkusić, Emir; Vytřas, Karel; Kalcher, Kurt Abstract: An amperometric biosensor was developed from screen printed carbon electrodes modified with magnetite as the transducer element and Nafion-entrapped glucose-oxidase as the bio-recognition layer, cast by drop-evaporation. In a flowing stream of phosphate buffer (pH 7.5/0.05M), at a working potential of-125mV vs. Ag/AgCl with an, injection volume of 100 ul, the biosensor has linear range of 7-150 mg l-1 glucose, a sensitivity of 3.8 nA mg-1 1 cm2, and a precision of 2% at 10 mg l-1. Uric acid, xanthine and hypoxanthine at 10 and 1000 mg l-1 do not cause significant error relative to 100 mg l-1 glucose. Ascorbic acid at 100 mg l-1 causes 10-14% deviations- Dilute blood samples showed average recovery rate of 95%. Thus this model biosensor is precise, fairly senstitive and accurate.

2006-01-01T00:00:00Z Miketová, Petra Moore, Ida M. (Ki) Hockenbery, Marilyn Khailová, Ludmila Jelínek, David Kernag, Casey Schram, Karl H. Hutter, John Kaemingk, Kristine L http://hdl.handle.net/10195/32747 2012-02-12T06:01:11Z 2006-01-01T00:00:00Z

Title: Applications of analytical techniques to clinical practice : assessment of chemical changes in patients with acute lymphoblastic leukemia following methotrexate administration Authors: Miketová, Petra; Moore, Ida M. (Ki); Hockenbery, Marilyn; Khailová, Ludmila; Jelínek, David; Kernag, Casey; Schram, Karl H.; Hutter, John; Kaemingk, Kristine L Abstract: The application of the following methods was described for the purpose of exploration of different cerebrospinal fluid biomarkers related to the cognitive impairment in children suffering from acute lymphoblastic leukemia and receiving methotrexate as part of the treatment regimen: gas chromatography/mass spectrometry was employed to measure the levels of homovanillic acid and vanilmandelic acid: twodimensional electrophoresis was used to find the changes in protein profiles present in cererebrospinal fluid: and high performance liquid chromatography was utilized to measure the levels of cerebrospinal fluid ceramides. The overall goal of this project was to utilize different analytical techniques in order to monitor chemical changes in cerebrospinal fluid associated wuth the administered amount of methotrexate.

2006-01-01T00:00:00Z